Abstract: Mesoporous silica molecular sieve (denoted MDL) has been prepared by the hydrolysis and condensation of tetraethylorthosilicate (TEOS) in the presence of industrial production N-dodecyl-1, 3-diaminopropane surfactant, which act as the structure-directing (templating) agent, through a supramolecular assemble mechanism between the gemini surfactant and Si(OC2H5)4-x(OH)x silica precursors. The mesoporous molecular sieve has vesicle-like particle texture and lamellar framework, as confirmed by Scanning electron microcopy (SEM) and Transmission electron microcopy (TEM). The most prior preparation condition is mentioned from the influence of preparation conditions to as-synthesized samples by X-ray power diffaration (XRD). FT-IR spectra show the existence of the mesoporous silica framework.