Abstract: Effects of contents of Na, Pt and Zr, and preparation of Pt-ZrO2/HM catalyst on n-pentane isomerization reaction were investigated in a continuous microreactor-chromatographic unit. A mathematical orthogonal experimental method was used to obtain an optimum reaction conditions: t=275 ℃, p=1.5 MPa, WHSV=1.25 h－1, H2/Ｃ50=2.0 (mole ratio). At the optimum conditions, Pt-ZrO2/HM catalyst achieved that n-pentane conversion is 69.88w%, isopentane yield is 67.68w%, isopentane selectivity is 96.85w%, yield of liquid product is 97.80w%.